BST DNA POLYMERASE, LARGE FRAGMENT
BST DNA聚合酶，大片段
Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5´ → 3´ polymerase activity, but lacks 5´ →3´ exonuclease activity. Enzyme provide strong strand displacement activity. The optimum temperature is from 60-65°C. Bst becomes heat inactivated at 80°C. Bst Polymerase, Large Fragment is prepared from an E. coli strain containing a gene of the Bacillus stearothermophilus DNA Polymerase gene, lacking the 5´ → 3´ exonuclease domain. Bst DNA聚合酶，大片段是嗜热脂肪芽孢杆菌DNA聚合酶蛋白质中包含5´的部分→ 3´聚合酶活性，但缺乏5´→3´核酸外切酶活性。酶提供强大的链置换活性。最适温度为60-65°C。Bst在80°C下热失活。Bst聚合酶，从含有嗜热脂肪芽孢杆菌DNA聚合酶基因的大肠杆菌菌株中制备大片段，缺少5´的→ 3´核酸外切酶结构域。
Storage condition: -20о C in 10 mM Tris-HCl (pH 8.0 at 25оС), 10 mM KCl, 1% BSA, 0,02% Tween 20, 50% glycerol. -在10 mM Tris-HCl（25ОС时pH值为8.0）、10 mM KCl、1%BSA、0.02%吐温20、50%甘油中加入20ОC。
Unit definition: One unit is defined at the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C. 一个单位定义为在65°C下30分钟内将10 nmol dNTP并入酸不溶性物质的酶量
Quality control: Each lot of enzyme is tested for endonuclease and non specific exonuclease activity. 每批酶都要进行核酸内切酶和非特异性核酸外切酶活性测试。
Applications:
• LAMP 灯具
• Reverse transcription 反转录
• Whole Genome Amplification 全基因组扩增
CAS9 NUCLEASE
The Cas9 protein is a recombinant endonuclease from Streptococcus pyogenes with a molecular weight of 150 kDa. Cas9 endonuclease in complex with RNA guides (crRNA duplex: tracrRNA) or single sgRNA catalyzes site-specific hydrolysis of the phosphodiester bond in double-stranded DNA. The cleavage is on the DNA chain between the third and fourth nucleotides from the
PAM sequence (NGG is a motif adjacent to the protospacer) with the formation of blunt ends. Recombinant Cas9 endonuclease does not contain a nuclear localization sequence.
Cas9蛋白是一种来自化脓链球菌的重组核酸内切酶，分子量为150 kDa。Cas9核酸内切酶与RNA导向物（crRNA双链：tracrRNA）或单链sgRNA复合物催化双链DNA中磷酸二酯键的位点特异性水解。切割发生在DNA链上，位于
PAM序列（NGG是与原间隔基相邻的基序）具有钝端的形成。重组Cas9核酸内切酶不含核定位序列。
Application: genomic editing, «CRISPR/Cas9» technology. 基因组编辑，«CRISPR/Cas9»技术。
Source: Cas9 endonuclease purified from E. coli strain containing a plasmid with the cloned full length gene of Cas9 Streptococcus pyogenes. 从含有克隆的化脓性链球菌Cas9全长基因质粒的大肠杆菌菌株中纯化的Cas9核酸内切酶。
Cas9 endonuclease concentration: 20 μM (20 pmol/μl). Cas9核酸内切酶浓度：20μM（20 pmol/μl）。
Total amount of Cas9 endonuclease: 300 pmol (15 μl). Cas9核酸内切酶总量：300 pmol（15μl）。
Storage buffer: 300 мМ NaCl, 50 мМ Tris-HCl, 0,1 мМ EDTA, 5 мМ β-mercaptoethanol, 50% glycerol (pH=7.5 @ 25°C).
300ММNaCl，50ММTris HCl，0,1МEDTA，5ММβ-巯基乙醇，50%甘油（25°C时pH=7.5）。
The optimum reaction temperature: 37°C. 最佳反应温度：37℃。
Enzyme inactivation: 65°C for 5 minutes. 酶失活：65°C持续5分钟。
Quality control: Each batch of the enzyme is tested for enzyme activity, electrophoretic purity, and the absence of non-specific endonuclease activity. 每批酶都要进行酶活性、电泳纯度和非特异性核酸内切酶活性的检测。
Storage and transportation: at -20°С. 储存和运输温度为-20°С。
CAS9-NLS NUCLEASE
CAS9-NLS核酸酶
The Cas9-NLS protein is a recombinant endonuclease from Streptococcus pyogenes fused from the C-terminus to the repeated nuclear localization signal (NLS) of the SV40 virus (PKKKRKV). The molecular weight of the Cas9-NLS protein is163 kDa. Cas9-NLS endonuclease in complex with RNA guides (crRNA duplex: tracrRNA) or single sgRNA catalyzes site-specific hydrolysis of the phosphodiester bond in double-stranded DNA (Figure). The cleavage is on the DNA chain between the third and fourth nucleotides from the PAM sequence (NGG is a motif adjacent to the protospacer) with the formation of blunt ends.
Cas9-NLS蛋白是一种由化脓链球菌产生的重组核酸内切酶，从SV40病毒（PKKKRKV）的C端融合到重复核定位信号（NLS）中。Cas9-NLS蛋白的分子量为163 kDa。Cas9 NLS核酸内切酶与RNA导向物（crRNA双链：tracrRNA）或单个sgRNA的复合物催化双链DNA中磷酸二酯键的位点特异性水解（图）。PAM序列的第三个和第四个核苷酸之间的DNA链（NGG是与原间隔基相邻的基序）发生断裂，形成钝端。
Application: genomic editing, «CRISPR/Cas9» technology. 基因组编辑，«CRISPR/Cas9»技术。
Source: Cas9 endonuclease purified from E. coli strain containing a plasmid with the cloned full length gene of Cas9 Streptococcus pyogenes. 从含有克隆的化脓性链球菌Cas9全长基因质粒的大肠杆菌菌株中纯化的Cas9核酸内切酶。
Cas9 endonuclease concentration: 20 μM (20 pmol/μl).
Cas9核酸内切酶浓度：20μM（20 pmol/μl）。
Total amount of Cas9 endonuclease: 300 pmol (15 μl).
Cas9核酸内切酶总量：300 pmol（15μl）。
Storage buffer: 300 мМ NaCl, 50 мМ Tris-HCl, 0,1 мМ EDTA, 5 мМ β-mercaptoethanol, 50% glycerol (pH=7.5 @ 25°C).
300ММNaCl，50ММTris HCl，0,1МEDTA，5ММβ-巯基乙醇，50%甘油（25°C时pH=7.5）。
The optimum reaction temperature: 37°C. 最佳反应温度：37℃。
Enzyme inactivation: 65°C for 5 minutes. 酶失活：65°C持续5分钟。
Quality control: Each batch of the enzyme is tested for enzyme activity, electrophoretic purity, and the absence of non-specific endonuclease activity. 每批酶都要进行酶活性、电泳纯度和无非特异性核酸内切酶活性的测试。
Storage and transportation: at -20°С. 储存和运输温度为-20°С。
